Muteesa I Royal University (MRU) is a private university in Uganda. It was accredited by the Uganda National Council for Higher Education (UNCHE) in 2007. On 15 June 2016, Justice Julia Sebutinde was installed as Chancellor of the University, replacing Ronald Muwenda Mutebi II, the founding chancellor who officially became The Visitor of the University.

The main campus is in the city of Masaka, approximately 135 kilometres (84 mi), by road, southwest of Kampala, Uganda's capital and largest city.

The second campus is on Mengo Hill, the seat of the Buganda Government, within the city of Kampala.

The third campus is in the town of Mubende, approximately 153 kilometres (95 mi), by road, west of Kampala.

MRU was established when ownership of the Masaka Technical Institute (Uganda Technical College, Masaka), was transferred from the government of Uganda to the Buganda government in January 2007.